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Description
YaxinBio Carboxypeptidase B Supplier Recombinant Carboxypeptidase B in E.coli
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Description
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Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli?and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Gene Name:Cpb2 carboxypeptidase B2 (plasma) [Rattus norvegicus] Synonyms:carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5 GeneID:113936 mRNA Refseq:NM_053617 Protein Refseq NP_446069 UniProt ID Q9EQV9 ?
Main Features
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Source | E. Coli |
Purified by | HPLC |
Purity | Single main band on SDS-PAGE |
M.W. | 33.8kD |
Contaminant activity | Less than 10 ppm of recombinant trypsin. |
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Unit? Definition
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One Unit of carboxypeptidase B activity hydrolyzes one micromole of??hippuryl-L-arginine per minute at 25℃, pH 7.65.
? Applications ? Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.
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Recommend? Usage
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To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH?7-9.
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Storage Instructions
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Recommend recombinant carboxypeptidase B lyophilized?should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90%?activity remained?after 10 times repeated freezing and thawing.
? Pathway ? Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Protein digestion and absorption, organism-specific biosystem ? Function ? carboxypeptidase activity; metallocarboxypeptidase activity; zinc ion binding
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Shanghai Yaxin Biotechnology Company
Recombinant Carboxypeptidase B,Human Trypsin,Recombinant Enterokinase
Address: No.466 YinDu Road,XuHui, Shanghai, China,
Shanghai, Shanghai
China, 20000
Tel: 86-21-54336592
Fax: 86-21-54336593
